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Title: Discovery of a nitric oxide-responsive protein in Arabidopsis thaliana
Authors: Zarban, Randa 
Vogler, Malvina 
Wong, Aloysius 
Eppinger, Joerg 
Al-Babili, Salim 
Gehring, Chris 
Issue Date: 2019
Publisher: Elsevier
Source: Zarban, R., Vogler, M., Wong, A., Eppinger, J., Al-Babili, S., & Gehring, C. (2019). Discovery of a nitric oxide-responsive protein in Arabidopsis thaliana. Molecules, 24(15), 2691.
Journal: Molecules 
Abstract: In plants, much like in animals, nitric oxide (NO) has been established as an important gaseous signaling molecule. However, contrary to animal systems, NO-sensitive or NO-responsive proteins that bind NO in the form of a sensor or participating in redox reactions have remained elusive. Here, we applied a search term constructed based on conserved and functionally annotated amino acids at the centers of Heme Nitric Oxide/Oxygen (H-NOX) domains in annotated and experimentally-tested gas-binding proteins from lower and higher eukaryotes, in order to identify candidate NO-binding proteins in Arabidopsis thaliana. The selection of candidate NO-binding proteins identified from the motif search was supported by structural modeling. This approach identified AtLRB3 (At4g01160), a member of the Light Response Bric-a-Brac/Tramtrack/Broad Complex (BTB) family, as a candidate NO-binding protein. AtLRB3 was heterologously expressed and purified, and then tested for NO-response. Spectroscopic data confirmed that AtLRB3 contains a histidine-ligated heme cofactor and importantly, the addition of NO to AtLRB3 yielded absorption characteristics reminiscent of canonical H-NOX proteins. Furthermore, substitution of the heme iron-coordinating histidine at the H-NOX center with a leucine strongly impaired the NO-response. Our finding therefore established AtLRB3 as a NO-interacting protein and future characterizations will focus on resolving the nature of this response.
DOI: 10.3390/molecules24152691
Appears in Collections:Scholarly Publications

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