Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12540/99
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dc.contributor.authorWee, Wei Y.en_US
dc.contributor.authorTan, Tze K.en_US
dc.contributor.authorJakubovics, Nicholas S.en_US
dc.contributor.authorChoo, Siew W.en_US
dc.date.accessioned2020-07-20T07:35:43Z-
dc.date.available2020-07-20T07:35:43Z-
dc.date.issued2016-
dc.identifier.citationWee, W. Y., Tan, T. K., Jakubovics, N. S., & Choo, S. W. (2016). Whole-genome sequencing and comparative analysis of Mycobacterium brisbanense reveals a possible soil origin and capability in fertiliser synthesis. PloS ONE, 11(3), e0152682.en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12540/99-
dc.description.abstractMycobacterium brisbanense is a member of Mycobacterium fortuitum third biovariant complex, which includes rapidly growing Mycobacterium spp. that normally inhabit soil, dust and water, and can sometimes cause respiratory tract infections in humans. We present the first whole-genome analysis of M. brisbanense UM_WWY which was isolated from a 70-year-old Malaysian patient. Molecular phylogenetic analyses confirmed the identification of this strain as M. brisbanense and showed that it has an unusually large genome compared with related mycobacteria. The large genome size of M. brisbanense UM_WWY (~7.7Mbp) is consistent with further findings that this strain has a highly variable genome structure that contains many putative horizontally transferred genomic islands and prophage. Comparative analysis showed that M. brisbanense UM_WWY is the only Mycobacterium species that possesses a complete set of genes encoding enzymes involved in the urea cycle, suggesting that this soil bacterium is able to synthesize urea for use as plant fertilizers. It is likely that M. brisbanense UM_WWY is adapted to live in soil as its primary habitat since the genome contains many genes associated with nitrogen metabolism. Nevertheless, a large number of predicted virulence genes were identified in M. brisbanense UM_WWY that are mostly shared with well-studied mycobacterial pathogens such as Mycobacterium tuberculosis and Mycobacterium abscessus. These findings are consistent with the role of M. brisbanense as an opportunistic pathogen of humans. The whole-genome study of UM_WWY has provided the basis for future work of M. brisbanense.en_US
dc.format.extent15 pagesen_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoengen_US
dc.publisherPublic Library of Science (PLOS)en_US
dc.relation.ispartofPLoS ONEen_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/-
dc.titleWhole-genome sequencing and comparative analysis of Mycobacterium brisbanense reveals a possible soil origin and industrial potential capability in fertiliser synthesisen_US
dc.typeArticleen_US
dc.rights.licenseAttribution-NonCommercial 4.0 International (CC BY-NC 4.0)en_US
dc.identifier.doi10.1371/journal.pone.0152682-
dc.subject.keywordsMycobacterium Brisbanenseen_US
dc.subject.keywordsWhole-genome Analysisen_US
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