Please use this identifier to cite or link to this item:
Title: The brassinosteroid receptor BRI 1 can generate cGMP enabling cGMP ‐dependent downstream signaling
Authors: Wheeler, Janet I. 
Wong, Aloysius 
Marondedze, Claudius 
Groen, Arnoud J. 
Kwezi, Lusisizwe 
Freihat, Lubna 
Vyas, Jignesh 
Raji, Misjudeen A. 
Irving, Helen R. 
Gehring, Chris 
Issue Date: 2017
Publisher: John Wiley & Sons, Inc.
Source: Wheeler, J. I., Wong, A., Marondedze, C., Groen, A. J., Kwezi, L., Freihat, L., ... & Gehring, C. (2017). The brassinosteroid receptor BRI 1 can generate cGMP enabling cGMP‐dependent downstream signaling. The Plant Journal, 91(4), 590-600.
Journal: The Plant Journal 
Abstract: The brassinosteroid receptor brassinosteroid insensitive 1 (BRI 1) is a member of the leucine‐rich repeat receptor‐like kinase family. The intracellular kinase domain of BRI 1 is an active kinase and also encapsulates a guanylate cyclase catalytic centre. Using liquid chromatography tandem mass spectrometry, we confirmed that the recombinant cytoplasmic domain of BRI 1 generates pmol amounts of cGMP per μg protein with a preference for magnesium over manganese as a co‐factor. Importantly, a functional BRI 1 kinase is essential for optimal cGMP generation. Therefore, the guanylate cyclase activity of BRI 1 is modulated by the kinase while cGMP , the product of the guanylate cyclase, in turn inhibits BRI 1 kinase activity. Furthermore, we show using Arabidopsis root cell cultures that cGMP rapidly potentiates phosphorylation of the downstream substrate brassinosteroid signaling kinase 1 (BSK 1). Taken together, our results suggest that cGMP acts as a modulator that enhances downstream signaling while dampening signal generation from the receptor.
DOI: 10.1111/tpj.13589
Appears in Collections:Scholarly Publications

Files in This Item:
File Description SizeFormat 
wku_schlrs_publcn_000036.pdf3.91 MBAdobe PDFThumbnail
Show full item record

Page view(s)

checked on Apr 2, 2023


checked on Apr 2, 2023

Google ScholarTM



Items in WIRE are protected by copyright, with all rights reserved, unless otherwise indicated.